Fig. 3
From: The Rac inhibitor HV-107 as a potential therapeutic for metastatic breast cancer
HV-107 does not significantly affect cell cycle progression. Flow cytometric analysis using propidium iodide as a nuclear marker was conducted to assess cell cycle progression in MDA-MB-231 (A) and MDA-MB-468 (D) cells. The DNA content was used to determine the percentage of cells in the G0/G1, S, and G2/M phases. Bar graphs represent the overall distribution of cells across different phases of the cell cycle. The relative expression of cyclin D1 and Bcl2 in MDA-MB-231 (B, C) and MDA-MB-468 (E, F) cells was examined using western blot analysis. The results were analyzed by comparing the fold change in expression relative to the control condition. N = 4. Means and error bars represent ± SEM, and significant differences from control (*) are shown (p < 0.05)