Fig. 2

Lentiviral expression in endothelial, megakaryocyte, myeloid and lymphoid cells. A, B The titration of LV-mWasabi (n = 4) (A) and LV-F8BDD (n = 3 to 7) (B) constructs containing the five promoters (EF1α, VEC, KDR, Gp and ITGA) illustrating the packaging efficiencies. C GFP expression in transduced ECs, megakaryocytes, myeloid and lymphoid cells detected under a fluorescent microscope. The left panels represent green fluorescent signals and the right panels were under bright field, at 20 × magnification. D Quantitative analysis based on mean fluorescence intensity (MFI) by flow cytometry showing GFP expression in ECs, megakaryocytes, myeloid and lymphoid cells (n = 3). The differences in characteristics between groups were analyzed using the one way Welch ANOVA tests with Games-Howell post hoc tests (A, D), and Kruskal–Wallis tests (B); *p < 0.05, **p < 0.01, ***p < 0.001, n.s., no significant difference