Fig. 1
From: PAX5-miR-142 feedback loop promotes breast cancer proliferation by regulating DNMT1 and ZEB1
miR-142-5p/3p was a direct target of PAX5. A The candidates of miR-142 upstream regulator by ALGGEN. B The correlation between PAX5 with miR-142-5p and miR-142-3p, respectively. C The expression level of PAX5 in breast cancer cell lines detected by western blot. D The expression levels of miR-142-5p/3p in breast cancer cells detected by qRT-PCR. E The construction of PAX5-overexpressed MDA-231 and SK-BR-3 cells verified by western blot. F The expression levels of miR-142-5p/3p in PAX5-overexpressed MDA-231 and SK-BR-3 cells detected by qRT-PCR. G The construction of PAX5-delepted ZR-75-1 and MCF-7 cells verified by western blot. H The expression levels of miR-142-5p/3p in PAX5-delepted ZR-75-1 and MCF-7 cells detected by qRT-PCR. I The putative binding sites of PAX5 on miR-142 promoter region. J The interaction of putative binding sites of miR-142 promoter with PAX5 analyzed by ChIP assay. K Three truncated length of putative binding sites (P1, − 500 to + 87; P2, − 180 to + 87; P3, − 90 to + 87) from miR-142 promoter were constructed. L The luciferase activity of PAX5 on mutated binding site of miR-142 promoter. *p < 0.05