Fig. 4
From: PAX5-miR-142 feedback loop promotes breast cancer proliferation by regulating DNMT1 and ZEB1
DNMT1 regulated PAX5 expression by ZEB1. A The putative binding of ZEB1 on PAX5 promoter region. B ChIP analysis of ZEB1 and PAX5 promoter in MDA-231 and MCF-7 cells. The interaction of ZEB1 and PAX5 promoter region detected by luciferase reporter assay (C), and co-IP analysis (D). E The fold change of DNMT1 on PAX5 promoter in ZEB1-delepted MDA-231 cells. The PAX5 expression level in DNMT1-overexpressed ZEB1-delepted MCF-7 cells (left), and in DNMT1-delepted ZEB1-delepted MDA-231 cells, detected by qRT-PCR (F) and western blot (G). *p < 0.05