Fig. 5
From: PAX5-miR-142 feedback loop promotes breast cancer proliferation by regulating DNMT1 and ZEB1
The existence of PAX5-miR-142-DNMT1/ZEB1 feedback loop. A The predicted binding sites of miR-142-5p on 3’UTR of DNMT1 and miR-142-3p on 3′UTR of ZEB1 by TargetScan. B The regulation of miR-142 on DNMT1 and ZEB1 detected by luciferase reporter assay. C The expression level of DNMT1 in miR-142-5p-overexpressed MDA-231 and SK-BR-3 cells (left), and in miR-142-5p-delepted MCF-7 and ZR-75-1 cells (right). D The expression level of ZEB1 in miR-142-3p-overexpressed MDA-231 and SK-BR-3 cells (left), and in miR-142-3p-delepted MCF-7 and ZR-75-1 cells (right) detected by qRT-PCR and western blot (F). E Pull-down assay by biotined-miR-142-5p/3p. G The relative mRNA expression levels of DNMT1 and ZEB1 in PAX-overexpressed, PAX5-overexpressed with miR-142-5p-delpeted, and PAX5-overexpressed with miR-142-3p-delpeted cells by qRT-PCR. H The expression levels of DNMT1 and ZEB1 in xenograft tumors. *p < 0.05