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Fig. 10 | Molecular Medicine

Fig. 10

From: HOXA1 participates in VSMC-to-macrophage-like cell transformation via regulation of NF-κB p65 and KLF4: a potential mechanism of atherosclerosis pathogenesis

Fig. 10

HOXA1 affects lipid accumulation, VSMC-to-macrophage transdifferentiation, and inflammation of POV-PC treated VSMCs via regulation of RelA and KLF4. The POV-PC treated VSMCs were infected with AAV1-shNC or AAV1-shHOXA1-1 together with RelA and KLF4 overexpression plasmid. A Oil-red O staining for lipids in POV-PC treated VSMCs. B Flow cytometry analysis and quantification for CD68+ cells. C Real-time qPCR assay for MAC2, MAC3, ABCA1, ACTA2, MYH11 and CNN1 expression. D ELISA for TNF-α and MMP-2 levels. Data are expressed as mean ± SD (n = 3). Ordinary one-way ANOVA followed by Tukey's multiple comparison test was used to calculate the P value in panel B–D. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658