Fig. 7

RT-qPCR validation of differentially expressed genes (DEGs) associated with morphological and physiological changes in female and male placentas after maternal LPS (400 µg/kg) exposure for 48 h

(A) RT-qPCR analysis of DEGs associated with trophoblast differentiation. (B) RT-qPCR analysis of DEGs that function as nutrient transporter involved in IUGR. (C) RT-qPCR analysis of DEGs categorized as female pregnancy. (D) RT-qPCR analysis of DEGs associated with immune system processes and ion transport. All experiments were performed in triplicate. The two-way anova was used for comparison between the PBS and LPS groups in females and males. Differences are represented as the mean ± standard error of mean (SEM). Significant differences were considered as *p < 0.05, **p < 0.01, ***p < 0.001