Fig. 4

A–C Levels of TNF-α (A), IL-6 (B), and IL-10 (C) in plasma were detected using double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) 24 h after CLP induction. D–F Double-antibody sandwich ELISA to detect the cytokines TNF-α (D), IL-6 (E), and IL-10 (F) in the mouse primary cells of each group after 6 h of LPS stimulation. Data are expressed as the mean ± SEM (n = 6). Significance levels are indicated as follows: ns, not significant; *p < 0.05, **p < 0.01, #p < 0.05, ##p < 0.01, &p < 0.05, &&p < 0.01