Fig. 6

A LC3 puncta marked the autophagosomes, showing that LPS-induced sepsis increased autophagosome expression. IFNγ treatment decreased sepsis autophagosome expression. Treatment with 2-DG increased autophagosome expression; hence, 2-DG and LY294002 inhibited the therapeutic effect of IFNγ. B The expression levels of Bcl-2 and Bax mRNA in splenocytes were detected via quantitative reverse transcription polymerase chain reaction. Data are expressed as the mean ± SEM (n = 3). C The TUNEL assay was performed to detect cell apoptosis. FITC staining (green) marks DNA fragmentation. Significance levels are indicated as follows: ns, not significant; *p < 0.05, **p < 0.01, #p < 0.05, ##p < 0.01, &p < 0.05, &&p < 0.01