Fig. 10
From: Nicotine exacerbates diabetic nephropathy through upregulation of Grem1 expression
Grem1 expression in the glomerular of mouse kidneys. Immunofluorescence staining was performed to determine the co-localization of Grem1 and Nephrin in mouse glomeruli. A Co-localizations of Grem1 and Nephrin in representative figures. The scale bar is 20 μm. B The average fluorescence intensity was measured per region of interest (ROI) for Grem1 or Nephrin, and the results (mean ± SD) represent 10 randomly and blindly selected regions. *(p < 0.05) compared with the Con group, and # (p < 0.05) compared with the DM group. Note: C, control; N, nicotine; D, diabetes mellitus; N + D, nicotine + diabetes mellitus