Fig. 7
From: Nicotine exacerbates diabetic nephropathy through upregulation of Grem1 expression
RNA-seq in the kidneys of Con and Nic, DM, Nic + DM mice were analyzed. A Differentially expressed genes (DEGs) in Nic + DM group vs. Con group. Red and blue dots represent up- and down-regulated mRNAs, respectively. Upregulated genes that log2FC > = 5 and − lg (p-value) > 5 or the down-regulated genes that log2FC < = − 5 and − lg (p-value) > 5 were listed. B Venn analysis of the top 50 genes with the highest fold up-regulation, all genes in cluster 2, and all expressed genes identified in the Nic and DM groups. C Fuzzy C-means clustering was performed to identify groups of particular genes responsive to nicotine-exacerbated DN in mice