Table 1 Principles and characteristics of different single cell sequencing
From: Single-cell RNA sequencing for the study of kidney disease
Methods | 10X Genomics Chromium System | Fluidigm C1 System | Illumina /BioRad |
|---|---|---|---|
Transcript coverage | 5’ /3’ end-counting | Full-length | 100 bp reads |
unique molecular identifiers (UMI) | Yes | No | Yes |
Single cell isolation | droplet-based microfluidic system | microfluidic-based system | Fluorescence-activated cell sorting |
Cell size limitations | Independent of cell size | Homogenous size of 5–10,10–17,or 17–25 µM | Independent of cell size |
Cell number required | ≥ 20,000 | ≥ 10,000 | Hundreds ~ thousands |
Throughput | High | Low | High |
Precision | High | Medium | High |
Cost | Low | High | Medium |
Operation | Easy | Difficult | Easy |
Run time (approximately 5000 single cells) | ~ 2–3 days | ~ 3 weeks | ~ 2 days |