Table 2 The studies on renal diseases using scRNA-seq
From: Single-cell RNA sequencing for the study of kidney disease
Diseases | Species | Cells or Samples | Cell Number | Device | Findings | Ref. |
|---|---|---|---|---|---|---|
LN | human | skin/kidney | 1584 single cell libraries | Fluidigm C1 | scRNA-seq can be used in skin biopsies to detect biomarkers of kidney disease | Der et al. 2017 |
| Â | human | skin/kidney | 250 cells of each type | Â | Heterogeneity and possible fibrotic pathways in LN | Der et al. 2019 |
| Â | human | kidney/urine /blood | 2881 cells | CEL-Seq2 | Detailed view of the active 21 leukocyte subpopulations in the kidney of a patient with LN | Arazi et al. 2019 |
RCC | human/ mouse | primary renal cell carcinoma/metastatic renal cell carcinoma | Â | Fluidigm C1 | Potential applications of scRNA-seq for precision anti-cancer therapy | Kim et al. 2016 |
| Â | human | primary renal cell carcinoma/metastatic renal cell carcinoma | 118 cells | Â | Heterogeneity of cancer cells in different subpopulations can activate different pathways and subdifferential markers in subpopulations are associated with intra-tumour heterogeneity, drug sensitivity and prognosis in renal cell carcinoma | Liu et al. 2021 |
| Â | human | primary tumour cells/lymph node metastatic tumour cells/bone metastatic tumour cells | 15,208 cells | 10X GenomicsChromium | Differentiation process of tumour stem cells and genes associated with poor prognosis in collecting ductal renal cell carcinoma | Pan et al. 2020 |
| Â | human | renal cell carcinoma benign adjacent renal tissue cells | 20,500 cells | 10X GenomicsChromium | Renal cell carcinoma benign adjacent renal tissue cells | Zhang et al. 2021 |
| Â | human | kidney | Â | 10X GenomicsChromium | Revealing and comparing neutrophil subpopulations in the healthy kidney and tumour microenvironment facilitates the understanding of the heterogeneity and pathological significance of neutrophils in kidney disease | Meng et al. 2021 |
|  | human | kidney |  | 10X GenomicsChromium | HIF-1α is mainly expressed in tumor-associated macrophages; HIF-2α and hypoxia-related factors are mainly expressed in tumor cells. HIF-1α can be used as a therapeutic target and disease progression marker in clear renal cell carcinoma | Cowman et al. 2020 |
| Â | human | Blood/ tumour/ renal parenchymal samples | 37,055 cells | 10X GenomicsChromium | Different transcriptional states of tumour-infiltrating CD8+ T cells reveal certain immune cell subsets vulnerable to therapeutic intervention | Borcherding et al. 2021 |
DN | mouse | glomerular cells | 644 cells | Fluidigm C1 | Unravelling the dynamics of gene expression in the diabetic kidney and the differential response of individual cells to diabetic injury | Fu et al. 2019 |
| Â | human | kidney cells/ immune cells | 21,529 nuclei | 10X GenomicsChromium | Cellular crosstalk in DN, the relationship between key genes for cellular communication and renal function | Wei et al. 2021 |
| Â | human | renal cortical cells | 23,980 nuclei | 10X GenomicsChromium | Single-cell transcriptome map of early DN in humans | Wilson et al. 2019 |
AKI | human | Urine | 30,076 cells | 10X GenomicsChromium | Differences in cell composition and gene expression during AKI, several inflammatory immune cell populations and differential activation pathways | Cheung et al. 2022 |
| Â | mouse | kidney | 26,643 cells | 10X GenomicsChromium | Gene expression changes during the repair of AKI, suggesting new therapeutic targets | Kirita et al. 2020 |
| Â | mouse | kidney | 54,730 cells | Droplet-based system | The first comprehensive renal cell type-specific transcriptional profile, potentially pathological epithelial interstitial crosstalk | Rudman-Melnick et al. 2020 |
| Â | mouse | glomerular cells | 75,000 cells | 10X GenomicsChromium | Comprehensive high-resolution single-cell transcriptomic profiles generated, providing a resource for identifying novel disease-associated genes and pathways | Chung et al. 2020 |
| Â | human/ mouse | Kidney cells/immune cells/epithelial cells | Â | 10X GenomicsChromium | Spatial transcriptome characterisation of AKI in a mouse model and demonstrates how the approach can be applied to human kidney tissue | Melo Ferreira et al. 2021 |
| Â | human | kidney | 23,367 cells | 10X GenomicsChromium | Predicted potential pathways of renal injury through angiotensin-converting enzyme 2 (ACE2) in 2019 coronavirus disease | He et al. 2020 |
| Â | mouse | kidney | 8732 cells | 10X GenomicsChromium | Ferroptosis-related genes were mainly expressed in tubular epithelial cells after I/R injury | Zhao et al. 2020 |
IgAN | human | kidney/ peripheral blood | 2785 Kidney cells/835 peripheral blood mononuclear cells | 10X GenomicsChromium | This study provided a promising prospect for disease treatment and suggested a role for CD8+ T-cell immune dysfunction in IgAN progression | Zheng et al. 2020 |
| Â | mouse | kidney | Â | Smart-seq2 | The role of endothelial cells in immune cell recruitment, paracrine pathways within the glomerulus associated with inflammation promotion | Zambrano et al. 2022 |
Glomerulonephritis | human | kidney | 14,932 cells | 10X GenomicsChromium | Podocyte markers of glomerulonephritis, specific markers of IgA, membranous nephropathy and LN at the cellular level | Chen et al. 2021 |
Other | mouse | glomerular endothelial cells | 40,000 cells | 10X GenomicsChromium | High-resolution mapping of the renal endothelium, phenotypic heterogeneity of renal endothelial cells, responses and expression of endothelial cell involvement in hypertonic and dehydration responses | Dumas et al. 2020 |
| Â | human | healthy kidney Cells/transplanted kidney Cells | 4487 cells | InDrops | Heterogeneity of the immune response in mixed rejection reactions | Wu et al. 2018 |
| Â | mouse | kidney | 57,979 cells | Droplet-based system | Most genetic disorders of the kidney can be traced back to a single cell type. the relationship between the renal collecting duct system and diseases such as metabolic acidosis, chronic kidney disease and blood pressure | Park et al. 2018 |