Fig. 2

Knockdown of P300 reduced cell viability and increased cell death of HASMCs with CD- and IKE-induced ferroptosis. A-B. The protein level of P300 were detected by western blot in total and nuclear protein extraction of HASMCs infected with lenti-shRNA or lenti-shP300. (n = 4 per group). (A) Total protein extraction western blot; (B) Nuclear protein extraction. C. Representative images showing cell death induced by CD, IKE, alone or in combination with Fer-1 after HASMCs were infected with lenti-shRNA, lenti-shP300-1 and lenti-shP300-2. D-E. Relative viability of the indicated lentivirus-infected HASMCs evaluated by CCK8 after treatment with CD (D), IKE (E), alone or in combination with Fer-1 (n = 6 per group). F. Relative cell death of HASMCs infected with the indicated lentivirus evaluated by LDH after the treatment described above (n = 5 per group). G-H. Percentage of PI positive indicated lentivirus-infected HASMCs examined by flow cytometry with propidium iodide (PI) staining after treatment as described above (n = 4 per group). Values are means ± SD; ***p < 0.001, NS, no significant