Fig. 3

Downregulation of P300 aggravated the accumulation of lipid peroxidation during HASMC ferroptosis. A-D. The level of lipid ROS (oxidized BODIPY-C11/non-oxidized BODIPY-C11 fluorescence ratio) examined by using BODIPY-C11 kit after treatment with CD (A-B) and IKE (C-D), alone or in combination with Fer-1, in HASMCs infected with lenti-shRNA, lenti-shP300-1 and lenti-shP300-2 (n = 4 per group). E and G. Representative images of immunofluorescence staining of 4-HNE in the indicated lentivirus-infected HASMCs treated with CD (E), IKE (G), alone or in combination with Fer-1. F and H. Quantitative analysis of 4-HNE (n = 4 per group). I-J. The level of MDA was evaluated by an MDA assay kit in indicated lentivirus-infected HASMCs after CD (I), IKE (J), or in combination with Fer-1 stimulation for the indicated time (n = 4 per group). Values are means ± SD; ***p < 0.001, **p < 0.01, *p < 0.05, NS, no significant