Fig. 4

A-485 reduced cell viability and increased cell death of HASMCs treated with CD and IKE. A. A CCK8 assay was performed to show the relative cell viability detected in HASMCs after treatment with different concentrations of A-485 (0, 0.5, 1, 2.5, 5, 10, 15 and 20 µM) (n = 6 per group). B-C. H3K27ac, H3K18ac, and H2BK5ac protein levels in HASMCs treated with A-485 at different concentrations of 0, 0.5, 1, 2.5, 5 and 10 µM were measured by western blot (n = 4 per group). β-actin served as a loading control. D. Representative images showing cell death after treatment with DMSO and A-485 under the ferroptosis models induced by CD and IKE, alone or in combination with Fer-1. E-F. The CCK8 assay showed the relative viability of HASMCs treated with DMSO and A-485 after CD (E), IKE (F), alone or in combination with Fer-1 stimulation for the indicated time (E: n = 4 per group, F: n = 5 per group). G. An LDH assay was performed to examine the cell death of HASMCs after treating as described above (n = 5 per group). H-I. Flow cytometry with PI staining showed the percentage of PI positive cells after treatment with DMSO and A-485 under the ferroptosis models induced by CD (H), IKE (I), alone or in combination with Fer-1 (n = 4 per group). Values are means ± SD; ***p < 0.001, NS, no significant