Fig. 5

A-485 aggravated the accumulation of lipid peroxidation during HASMC ferroptosis. A-D. The ratio of oxidized BODIPY-C11/non-oxidized BODIPY-C11 fluorescence detected by the BODIPY-C11 kit after treatment with DMSO and A-485 under the ferroptosis models induced by CD (A-B), IKE (C-D), alone or in combination with Fer-1 (n = 3 per group). E and G. Representative images of 4-HNE immunofluorescence staining in HASMCs treated with DMSO and A-485 after CD (E), IKE (G), alone or in combination with Fer-1 stimulation for the indicated time. F and H. Quantitative analysis of 4-HNE immunofluorescence staining (n = 4 per group). I-J. The level of MDA in HASMCs treated as described above was examined by an MDA assay kit (n = 3 per group). Values are means ± SD; ***p < 0.001, **p < 0.01, *p < 0.05, NS, no significant