Fig. 7

HIF-1α downregulation abolished the pro-ferroptotic effects of P300 deficiency in HASMCs. P300 knockdown HASMCs were infected with lenti-shRNA and lenti-shHIF-1α, and then these HASMCs were used for subsequent experiments. A-B. Relative cell viability was evaluated by CCK8 kit in HASMCs after treatment with CD (A) and IKE (B) (n = 5 per group). C-F. The level of lipid ROS (oxidized BODIPY-C11/non-oxidized BODIPY-C11 fluorescence ratio) examined by the BODIPY-C11 kit in HASMCs treated with CD (C-D) and IKE (E-F) (n = 4 per group). G-H. An MDA assay was used to detect the MDA levels of HASMCs after treatment with CD (G) and IKE (H) (n = 4 per group). I-L. Representative images of immunofluorescence staining and quantitative analysis of 4-HNE in HASMCs among the indicated groups (n = 4 per group). Values are means ± SD; ***p < 0.001, **p < 0.01, *p < 0.05, NS, no significant