Fig. 8

HIF-1α inhibition eliminated the pro-ferroptotic effects of P300 deficiency in HASMCs. A-B. Relative cell viability of HASMCs infected with lenti-shRNA, lenti-shP300-1, and lenti-shP300-2 by using a CCK8 kit after treatment with CD (A) and IKE (B) in the presence or absence of BAY87-2243 (n = 4 per group). C-F. The oxidized BODIPY-C11/non-oxidized BODIPY-C11 fluorescence ratio was evaluated by BODIPY-C11 kit in the indicated lentivirus-infected HASMCs treated with DMSO and BAY87-2243 after CD (C-D) and IKE (E-F) stimulation for 16 h (n = 4 per group). G-H. An MDA assay was used to assess MDA levels in indicated lentivirus-infected HASMCs treated with DMSO and BAY87-2243 after CD (G) and IKE (H) stimulation for the indicated time (n = 4 per group). I-L. Representative images of immunofluorescence staining and quantitative analysis of 4-HNE in HASMCs among the indicated groups in the presence or absence of BAY87-2243 (n = 4 per group). Values are means ± SD; ***p < 0.001, **p < 0.01, *p < 0.05, NS, no significant