Fig. 2

Effects of DDR1 on collagen fiber arrangement and immune escape in breast cancer. Note: (A) RT-qPCR detected the expression levels of DDR1 in breast cancer cell lines (EO771, EMT6, and 4T1.2), * indicates P < 0.05, ** indicates P < 0.01 compared with the NMuMG cells; (B) RT-qPCR and Western blot detected the silencing efficiency of DDR1 in EO771 cells, * indicates P < 0.05, ** indicates P < 0.01 compared with the sh-NC group; (C) Schematic diagram of BC-MPS construction; (D) Masson staining detected the collagen fiber arrangement in each group of BC-MPS (×100); (E) Tumor volume of each group of breast cancer mice; (F) Photos of tumors taken from each group of mice; (G) Tumor weight of each group of breast cancer mice; (H) RT-qPCR detected the expression levels of DDR1 in each group of breast cancer mice tumor tissues; (I) Immunohistochemistry detected the content of Collagen IV in ECM of each group of breast cancer mice tumor tissues (×200); (J) Masson staining detected the collagen fiber arrangement in each group of breast cancer mice tumor tissues (×400); (K) Immunohistochemistry detected the number of CD8-positive cells in each group of breast cancer mice tumor tissues (×200); (L) Schematic diagram of CD8 + T cell migration experiment; (M) CD8 + T cell migration experiment detected the migration of CD8 + T cells in each group of breast cancer mice tumor tissues after decellularized ECM treatment. * indicates P < 0.05, ** indicates P < 0.01 compared with the control group. All cell experiments were repeated 3 times; each group consisted of 5 mice