Fig. 4

Effects of TFAP2A-mediated DDR1 regulation on collagen fiber arrangement and immune cell infiltration in breast cancer. Note: (A) RT-qPCR detected the expression levels of TFAP2A and DDR1 in each group of EO771 cells; (B) Masson staining detected the collagen fiber arrangement in each group of BC-MPS treated with different EO771 cells (×100); (C) Tumor volume of each group of breast cancer mice; (D) Tumor weight of each group of breast cancer mice; (E) Photos of tumors taken from each group of mice; (F) RT-qPCR detected the expression levels of TFAP2A and DDR1 in each group of breast cancer mice tumor tissues; (G) Immunohistochemistry detected the content of Collagen IV in ECM of each group of breast cancer mice tumor tissues (×200); (H) Masson staining detected the collagen fiber arrangement in each group of breast cancer mice tumor tissues (×400); (I) Immunohistochemistry detected the number of CD8-positive cells in each group of breast cancer mice tumor tissues (×200); (J) CD8 + T cell migration experiment detected the migration of CD8 + T cells in each group of breast cancer mice tumor tissues after decellularized ECM treatment. * indicates P < 0.05 compared with the control group. All cell experiments were repeated 3 times; each group consisted of 5 mice