Fig. 6

HNRNPC regulates the effect of the TFAP2A/DDR1 axis on collagen fiber arrangement and immune cell infiltration by recognizing the m6A modification of TFAP2A mRNA by VIRMA. Note: (A) RT-qPCR detection of the expression levels of VIRMA, HNRNPC, TFAP2A, and DDR1 in each group of EO771 cells; (B) Masson staining detection of collagen fiber arrangement in each group of EO771 cells in BC-MPS (×100); (C) Tumor volume of each group of breast cancer mice; (D) Picture of tumor harvested from each group of breast cancer mice; (E) Tumor weight of each group of breast cancer mice; (F) RT-qPCR detection of the expression levels of VIRMA, HNRNPC, TFAP2A, and DDR1 in each group of breast cancer mice tumor tissue; (G) Immunohistochemistry detection of Collagen IV content in ECM of each group of breast cancer mice tumor tissue (×200); (H) Masson staining detection of collagen fiber arrangement in each group of breast cancer mice tumor tissue (×400); (I) Immunohistochemistry detection of CD8 positive cell number in each group of breast cancer mice tumor tissue (×200); (J) CD8 + T cell migration experiment to detect the number of CD8 + T cell migration in each group of breast cancer mice tumor tissue after decellularized ECM treatment. * indicates P < 0.05 when compared with the two groups. All cell experiments were repeated 3 times with 5 mice in each group