Fig. 6
Reactivation of Wnt signalling in the HSPP group alleviated the anhedonic behaviour and abnormal synaptic transmission induced by hormone withdrawal. a The schedule for administration with AMBMP on the HSPP model and the arrangement behavior tests. The HSP and HSPP group were injected with the same volume of a vehicle solvent with the AMBMP working solution to set as control. SPT test was performed after the 3-day administration of AMBMP. (HSP, n=9; HSPP, n=9; HSPP+AMBMP, n=9). b The sucrose preference test (SPT) was measured for two hours in the three treatment groups. c, d Recordings of sEPSCs and sIPSCs from acute brain slices of the HSP, HSPP, and HSPP+AMBMP groups. Representative traces (left), cumulative distribution plots, and bar graphs showed the amplitude (left) and frequency (right) of sEPSCs, and sIPSCs. (For all comparisons, n=8 neurons from 3 rats/group). e Relative expression of β-catenin and sFRP1 on hippocampus from three model groups with the 3-day treatment. f, g Relative expression of the γ2, δ, and α5 subunits of GABAARs in total and membrane hippocampal protein level in 3 groups. h, i Relative expression of p-GluA1(Ser845), GluA1, and PSD95 in the hippocampus in three treatment groups. j The expression of p-GluA1 (Ser845)/GluA1 ratio in two treatment regimens. All the expression of the protein was standardized by the HSP group. All data were assessed with One-way ANOVA. Tukey's multiple comparisons test was performed in comparison of the HSP versus the HSPP group, HSP versus the HSPP+AMBMP group, and the HSPP versus the HSPP+AMBMP group. Only significant comparisons were displayed in the figures. Data represent means ± SEM. *p<0.05, **p<0.005