Fig. 2
From: SBP1 promotes tumorigenesis of thyroid cancer through TXN/NIS pathway
SBP1 promotes cell growth and tube formation of thyroid cancer cell lines. FTC133 cells were transfected with the SBP1 overexpression vector, while BHT101 cells were transfected with the SBP1 siRNA. (A) Western blot assays were performed to assess transfection efficiency of SBP1 overexpression in FTC133 cells. (B) FTC133 cell viability was analyzed by CCK-8 assay. (C) FTC133 cells were transfected with SBP1 overexpression vector and the culture supernatant was cultured with human retinal microvascular endothelial cells (RCECs). Then tube formation was analyzed by tube formation assay. (D) Western blot assays were performed to assess transfection efficiency of SBP1 knockdown in BHT101 cells. (E) Cell viability was analyzed by CCK-8 assay. (F) BHT101 cells were transfected with SBP1 siRNA and the culture supernatant was cultured with RCECs, then tube formation was analyzed by tube formation assay. **P < 0.01, vs. negative control (NC), #P < 0.05, # #P < 0.01, vs. siRNA negative control (si-NC).