Fig. 3
From: SBP1 promotes tumorigenesis of thyroid cancer through TXN/NIS pathway
SBP1 promotes dedifferentiation of thyroid cancer cell lines. FTC133 cells were transfected with SBP1 overexpression vector, while BHT101 cells were transfected with SBP1 siRNA. (A) qRT-PCR assay were performed to assess mRNA levels of thyroid transcription factor-1 (TTF-1), thyroid transcription factor-1 (TTF-2), thyroid stimulating hormone receptor (TSH-R), thyroglobulin (TG) and sodium/iodide symporter (NIS) in FTC133 cells. (B) Western blot assays were performed to assess protein expression of TSH-R, TG and NIS in FTC133 cells. (C) Quantity analysis of protein expression of TSH-R, TG and NIS in FTC133 cells. (D) Immunocytochemical staining of NIS in FTC133 cells transfected with SBP1 overexpression vector. Scale bars: 50 μm. (E) qRT-PCR assay were performed to assess mRNA levels of TTF-1, TTF-2, TSH-R, TG and NIS in BHT101 cells. (F) Western blot assays were performed to assess protein expression of TSH-R, TG and NIS in BHT101 cells. (G) Quantity analysis of protein expressions of TSH-R, TG and NIS in BHT101 cells. (H) Immunocytochemical staining of NIS in BHT101 cells transfected with SBP1 overexpression vector. Scale bars: 50 μm. (I) NIS expression was determined by Western blot analysis in 12 pairs of primary thyroid cancer and their matched noncancerous thyroid tissues. (J) SBP1 was negatively correlated with NIS expression in 12 thyroid cancer tissues. *P < 0.05, **P < 0.01, vs. negative control (NC), #P < 0.05 vs. siRNA negative control (si-NC).