Fig. 5
From: SBP1 promotes tumorigenesis of thyroid cancer through TXN/NIS pathway
Knockdown of SBP1 inhibits xenograft tumor growth. BHT101 cells were transfected with si-SBP1 with or without TXN overexpression plasmid. (A) Western blot assays were performed to assess protein expression of NIS. (B) The cell viability was determined by CCK-8 assay. (C) Tube formation was analyzed by tube formation assay. (D) Tumor tissues from sh-SBP1 and sh-NC mice were homogenate and lysed. The protein levels of SBP1, TXN, TSH-R, TG, and NIS were analyzed by Western blot. (E) Quantity analyze of protein expressions of SBP1 and TXN. (F) Quantity analyze of protein expressions of TSH-R, TG, and NIS. (G) Photographs of dissected tumors from nude mice. (H) Tumor growth curves were compared between thyroid cells transfected by sh-SBP1 and sh-NC cells in nude mice. (I) Histogram represents mean tumor weight of dissected tumors from SBP1-knockdown and sh-NC mice, respectively. *P < 0.05 vs. si-SBP1 + NC; &P < 0.05, &&P < 0.01