Fig. 1

Ndufc2 silencing leads to cardiac hypertrophy and impairs NAD + metabolism and SIRT3 downstream pathway in H9c2 cells. H9c2 cells were silenced for Ndufc2 (Ndufc2-KD) for 48 h. A. Representative images of β-actin fluorescence and relative quantification are shown. B. Relative quantification by RTqPCR of Nppa and β-Mhc mRNA levels. C. NAD+:NADH ratio. D. Representative western blot for SIRT3 and corresponding quantification. E. Representative western blot for phospho-AMPK (Thr-172) and corresponding quantification. F. Representative western blot for phospho-AKT (Ser-473) and corresponding quantification. G. Representative western blot for MnSOD and corresponding quantification. H. Determination of intracellular reactive oxygen species (ROS). CTR indicates not silenced cells (cells incubated with lipofectamine + scramble siRNA). Values are expressed as mean ± SEM (N = 3–4) *p < 0.05; **p < 0.01, ***p < 0.001 obtained by using the student T test