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Fig. 7 | Molecular Medicine

Fig. 7

From: Leptin/obR signaling exacerbates obesity-related neutrophilic airway inflammation through inflammatory M1 macrophages

Fig. 7

The effects of leptin/obR signaling on neutrophil chemotaxis. a Real-time PCR of CXCL2 mRNA levels normalized to those of GAPDH. b CXCL2 protein levels were analyzed by ELISA. c Real-time PCR of CXCL2 mRNA levels normalized to those of GAPDH. d CXCL2 protein levels were analyzed by ELISA. e Transwell assay to detect neutrophil chemotaxis (×100). f Chemotactic index of neutrophils. g Transwell assay to detect neutrophil chemotaxis (×100). h Chemotactic index of neutrophils. Each experiment was performed three times. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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Molecular Medicine

ISSN: 1528-3658