Fig. 2

Effect of CLP ± exogenous insulin on the abundance of Phospho (p)IRβ and pIRS-1 in Skeletal Muscle and pIRβ and pIRS-2 in Liver. T₀: unoperated control; subscript indicated time (hrs) post-CLP. Superscript; –/+ indicates without or with insulin. Each point represents a measurement in a single animal, heavy bars − mean, light bars − ± standard deviation. Measurements performed using ELISA. Value at T₀ arbitrarily set at 1, other values normalized to T₀ value. Significance determined using two-way ANOVA with Tukey’s post-hoc test for multiple comparisons. *Statistically significant relative to intensity of animals receiving the same treatment (with or without insulin) at T0, ^Statistically significant relative to intensity at the same time point without insulin. For clarity, other significant differences (eg, relative to T₂₄ w/o insulin) not indicated. A Effect of CLP on tyrosine phosphorylation of IRβ in skeletal muscle without or in the presence of exogenous insulin. N = 3–5 for each group. B Effect of CLP on tyrosine phosphorylation of IRβ in liver without or in the presence of exogenous insulin. N = 3 for each group. C Effect of CLP on tyrosine phosphorylation of IRS-1 in skeletal muscle without or in the presence of exogenous insulin. N = 4 for each group. D Effect of CLP on tyrosine phosphorylation of IRS-2 in liver without or in the presence of exogenous insulin. N = 4 for each group