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Fig. 5 | Molecular Medicine

Fig. 5

From: Role of ATF3 triggering M2 macrophage polarization to protect against the inflammatory injury of sepsis through ILF3/NEAT1 axis

Fig. 5

ILF3 is required for the stabilization of NEAT1 through direct interaction. a, b The levels of NEAT1 and ILF3 in RAW264.7 cells were detected by qRT-PCR. The interaction between ILF3 and NEAT1 in RAW264.7 cells was assessed by RIP (c) and RNA pull-down assays (d). Normal IgG served as negative control for RIP assay. Antisense RNA acted as a negative control for RNA pull-down assay. e The mRNA level of ILF3 in RAW264.7 cells was detected by qRT-PCR. f Nascent NEAT1 was evaluated by nascent RNA capture assay. g The stability of NEAT1 in RAW264.7 cells was assessed by RNA stability assay. ***, P < 0.001

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Molecular Medicine

ISSN: 1528-3658