Fig. 9

The regulatory network of ATF3/ILF3/NEAT1 in the in vivo model of sepsis. a Survival analysis of mice in different groups. b Analysis of total protein concentration, numbers of total cells, neutrophils and macrophages in BALF. c Lung W/D ratio evaluation. d, e The levels of cytokines in BALF and lung tissues were measured by ELISA assay. f Histological changes of lung tissues were examined by H&E staining. Cell apoptosis in lung tissues was assessed by TUNEL assay. The immunoreactivity of CD163 was detected by IHC. The mRNA (g) and protein (h) levels of Arg-1 and iNOS in lung tissues were detected by qRT-PCR and western blot, respectively. i The level of NEAT1 was detected by qRT-PCR. j The protein levels of ATF3 and ILF3 were detected by western blot. *, P < 0.05, **, P < 0.01, ***, P < 0.001