Fig. 4

Effects of AQP3 on LPS-induced inflammatory injury of gallbladder mucosal epithelial cells. A RT-qPCR to detect the mRNA expression of AQP3 in gallbladder mucosal epithelial cells. B, Flow cytometry to detect the inflammatory injury of gallbladder mucosal epithelial cells. C, immunofluorescence to detect the inflammatory injury of gallbladder mucosal epithelial cells. Red fluorescence represents TUNEL, and blue fluorescence represents the nucleus. D, Trypan blue staining to observe the pyroptosis of gallbladder mucosal epithelial cells. E, ELISA to detect the expression of TNF-α, IL-1β, and IL-18 in gallbladder mucosal epithelial cells. All cell experiments were repeated 3 times. * p < 0.05