Fig. 6

Effects of AQP3 on LPS-induced inflammatory injury of gallbladder mucosal epithelial cells by regulating the AMPK/SIRT1 signaling pathway. LPS-induced gallbladder mucosal epithelial cells were treated with or-AQP3 alone or combined with an AMPK inhibitor. A Western blot to detect the protein expression and phosphorylation level of AMPK and protein expression of SIRT1 in gallbladder mucosal epithelial cells. B, Flow cytometry to detect inflammatory injury of gallbladder mucosal epithelial cells. C, TUNEL staining to detect inflammatory injury of gallbladder mucosal epithelial cells. D, Trypan blue staining to observe the pyroptosis of gallbladder mucosal epithelial cells. E, ELISA to detect the expression of TNF-α, IL-1β, and IL-18 in gallbladder mucosal epithelial cells. All cell experiments were repeated three times. * p < 0.05