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Fig. 7 | Molecular Medicine

Fig. 7

From: AQP3-mediated activation of the AMPK/SIRT1 signaling pathway curtails gallstone formation in mice by inhibiting inflammatory injury of gallbladder mucosal epithelial cells

Fig. 7

Effects of AQP3 on mice with cholelithiasis by regulating the AMPK/SIRT1 signaling pathway. A, RT-qPCR to detect the expression of AQP3 in the gallbladder tissues of mice with cholelithiasis. B, TUNEL staining to detect the pyroptosis of gallbladder mucosal epithelial cells in mice with cholelithiasis after AQP3 overexpression. Red fluorescence represents TUNEL, C, ELISA to detect the expression of TNF-α, IL-1β, and IL-18 in the gallbladder tissues of mice with cholelithiasis after AQP3 overexpression. D, RT-qPCR to detect the mRNA expression of SIRT1 in the gallbladder tissues of mice with cholelithiasis after AQP3 overexpression. E, Western blot to detect the protein expression and phosphorylation level of AMPK and protein expression of SIRT1 in the gallbladder tissues of mice with cholelithiasis after AQP3 overexpression. n = 10. * p < 0.05

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Molecular Medicine

ISSN: 1528-3658