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Fig. 7 | Molecular Medicine

Fig. 7

From: BAP1 mutations inhibit the NF-κB signaling pathway to induce an immunosuppressive microenvironment in uveal melanoma

Fig. 7

Effects of BAP1 mutations on the tumor immune microenvironment. A, The mRNA expression of BAP1 in the UM cells with WT or MUT BAP1 measured by RT-qPCR. B, The protein expression of BAP1 in the UM cells with WT or MUT BAP1 measured by Western Blot. C, ELISA detection of the expression of immune regulators (IL-1β, MCP1, TGF-β1 and CXCL10) in the in vitro co-culture system. D, RT-qPCR detection of the expression of macrophage-secreted cytokines (IL-1β, MCP1, TGF-β1 and CXCL10) after co-culture of UM cells with WT or MUT BAP1 and macrophages. E, MHCII expression on the macrophage surface by flow cytometry. F, The mRNA expression of MHCII alleles HLA-DRA and HLA-DRB on the surface of macrophages detected by RT-qPCR. WT, wild type; mutated BAP1, mutant type BAP1. * p < 0.05

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Molecular Medicine

ISSN: 1528-3658