Fig. 7

Metrnβ regulated the SIRT1-P53-SLC7A11 pathway to regulate ferroptosis. A Protein levels of Ferroportin, Ferritin, and Gpx4 in mice lung tissue treated with Ad-Metrnβ and challenged with LPS (n = 4). *P < 0.05 vs. the NC-CON group. #P < 0.05 vs. the NC-LPS group. B Protein levels of Ferroportin, Ferritin, and Gpx4 in mice lung tissue treated with Ad-shMetrnβ and challenged with LPS (n = 4). *P < 0.05 vs. the Ad-ScRNA-CON group. #P < 0.05 vs. the Ad-ScRNA-LPS group. C Protein levels of SIRT1, P53, and SLC7A11 in mice treated with Ad-Metrnβ and challenged with LPS (n = 4). *P < 0.05 vs. the NC-CON group. #P < 0.05 vs. the NC-LPS group. D Protein levels of SIRT1, P53, and SLC7A11 in mice lung tissue treated with Ad-shMetrnβ and challenged with LPS (n = 4). *P < 0.05 vs. the Ad-ScRNA-CON group. #P < 0.05 vs. the Ad-ScRNA-LPS group. E Luciferase analysis of MLE-12 cells co-transfected with pSIRT1-WT and Metrnβ siRNA or pSIRT1-Mut and Metrnβ siRNA challenged with LPS. *P < 0.05 vs. the ScRNA group. F ChIP analysis of MLE-12 cells challenged with LPS cultured with Metrnβ antibody. G Protein levels of SIRT1, P53, and SLC7A11 in mice lung tissue challenged with LPS (n = 4). *P < 0.05 vs. theWT-CON group; #P < 0.05 vs. the WT-LPS group