Fig. 2

AGEs promote LDL transcytosis and uptake in HUVECs. A Schematic diagram of FITC-LDL transcytosis across endothelial cells. B LDL transcytosis in HUVECs under different concentrations (25 µg/mL, 50 µg/mL, 100 µg/mL) of BSA or AGEs treatment; n = 3. C LDL transcytosis in HUVECs under different times (6 h, 12 h, 24 h) of BSA or AGEs treatmen; n = 3. D, E HUVECs were treated with vehicle, BSA or AGEs (100 μg/mL, 24 h), followed by incubation with 50 μg/mL DiI-LDL. D Representative confocal fluorescent images showing DiI-LDL uptake in HUVECs. The red signals denote DiI-LDL puncta, and the blue DAPI signals represent nuclei (n = 3). Scale bars: 20 μm. E Cells were harvested, and the MFI of DiI-LDL in cells was measured by flow cytometry analyses. F Quantification of DiI-LDL uptake by HUVECs; n = 3. G Quantification summary of DiI-LDL uptake represented by MFI in HUVECs; n = 3