Fig. 4

RAGE is involved in AGEs-regulated Caveolin-1 mediated LDL uptake. A, B Dose–response (left top) and time course (right top) of RAGE protein induction by AGEs. C, D Summary bar graphs showing the expression of the indicated proteins; n = 3. E, F Cells were transfected with either control or RAGE siRNA for 48 h and then treated with AGEs for 24 h. Representative western blotting analyses of the indicated proteins E and summary bar graph showing the expression of the indicated proteins (F). G–H HUVECs were transfected with either control or RAGE siRNA for 48 h and then treated with AGEs for 24 h, followed by incubation with 50 μg/mL DiI-LDL. G Representative confocal fluorescent images showing DiI-LDL uptake in HUVECs. The red signals denote DiI-LDL puncta, and the blue DAPI signals represent nuclei. Scale bars: 20 μm. H Cells were harvested, and the MFI of DiI-LDL in cells was measured by flow cytometry analyses. I Quantification of DiI-LDL uptake by HUVECs (n = 3). J Quantification summary of DiI-LDL uptake represented by MFI in HUVECs (n = 3)