Fig. 6

RAGE silencing alleviated diabetic AS in ApoE^−/− mice. A Schematic diagram of the animal experimental process. B The representative images of immunohistochemistry (IHC) staining for RAGE, Caveolin-1, phosphorylated p65 (p-p65), NF-κB (p65) and IκBα protein expression. C Representative micro pictures of the aortic arches from ApoE^−/− mice. Arrows and dashed lines indicate atherosclerotic plaques in the brachiocephalic artery, subclavian artery and lesser curvature of the aortic arch. D The percentage of aortic arch lesion area in the indicated group (n = 9). E, F Representative histological analysis of brachiocephalic arteries isolated from ApoE^−/− mice and stained with HE and the percentage of positively regions in the indicated groups (n = 9). G, I Representative images of Oilred O-stained aortic root sections and the lesion area in the indicated groups (n = 9). Scale bars: 500 μm. H, J Representative images of aorta sections with Oil Red O-stained aortas (en face) and the percentage of positively regions in the indicated groups (n = 9)