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Fig. 2 | Molecular Medicine

Fig. 2

From: Quercetin inhibits the metabolism of arachidonic acid by inhibiting the activity of CYP3A4, thereby inhibiting the progression of breast cancer

Fig. 2

Effects of quercetin on proliferation and apoptosis of breast cancer cells. A MTS assay was used to determine the viability of breast cancer cells in each group; B CCK-8 assay was used to determine the proliferation of breast cancer cells in each group; C colony formation assay was used to determine the colony formation ability of breast cancer cells in each group; D, E flow cytometry was used to determine the cell cycle of breast cancer cells in each group; F flow cytometry was used to determine the apoptosis of breast cancer cells in each group; G Western blot was used to determine the protein expression of proliferation- and apoptosis-related factors in breast cancer cells of each group. The data results are represented as mean ± standard deviation for quantitative data. Independent-sample t-test was used to analyze two groups, one-way ANOVA was used to analyze multiple groups, and Tukey’s post hoc test was used for comparison. The cell experiments were repeated 3 times. * indicates P < 0.05 compared with the Quercetin 0 μM group, ** indicates P < 0.01, *** indicates P < 0.001, and **** indicates P < 0.0001

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