Fig. 3

The effect of Quercetin on the adhesion, migration, and invasion of breast cancer cells. A Cell adhesion experiment detecting the adhesion of different groups of breast cancer cells; B Transwell experiment detecting the invasion ability of different groups of breast cancer cells; C Transwell experiment detecting the migration ability of different groups of breast cancer cells; D Immunofluorescence detects the expression of paxillin and Ac-tubulin in different groups of breast cancer cells; E Western blot examine the protein expression of MMP2, MMP9, TIMP1, and TIMP2 in different groups of breast cancer cells; F Gelatin zymography analyses the protein expression of activated MMP2 and MMP9 in different groups of breast cancer cells. (The data results are quantitative and represented by mean ± standard deviation. An independent sample t-test was used for analysis between two groups, and a one-way analysis of variance was used for analysis among multiple groups. Tukey’s test was used for post-hoc analysis. The cell experiment was repeated 3 times, *** represents P < 0.001 compared to Quercetin 0 μM, and **** represents P < 0.0001.)