Skip to main content
Fig. 4 | Molecular Medicine

Fig. 4

From: Quercetin inhibits the metabolism of arachidonic acid by inhibiting the activity of CYP3A4, thereby inhibiting the progression of breast cancer

Fig. 4

Quercetin affects AA metabolism by regulating the expression and activity of CYP3A4 in breast cancer cells. A Potential targets of Quercetin screened from CTD and STITCH databases, with inner circle red genes representing common targets between CTD and STITCH databases, and outer circle green representing targets selected only from CTD database; B Western blot detection of CYP3A4 protein expression in various breast cancer cell groups treated with Quercetin; C Detection of CYP3A4 activity in various breast cancer cell groups using Midazolam as the probe substrate; D LC–MS/MS detection of EETs content in various breast cancer cell groups; E LC–MS/MS detection of AA content in various breast cancer cell groups. The results are expressed as means ± standard deviation and analyzed by independent sample t-test for two groups and one-way ANOVA with Tukey’s post-hoc test for multiple groups. The experiments were repeated three times, and * indicates P < 0.05 compared with the Quercetin 0 μM group, ** indicates P < 0.01, *** indicates P < 0.001, and **** indicates P < 0.0001

Back to article page

Molecular Medicine

ISSN: 1528-3658