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Fig. 5 | Molecular Medicine

Fig. 5

From: Quercetin inhibits the metabolism of arachidonic acid by inhibiting the activity of CYP3A4, thereby inhibiting the progression of breast cancer

Fig. 5

Quercetin regulates the phosphorylation and nuclear translocation of Stat3 through EETs in breast cancer cells. A Western blot detects the expression of Stat3 and p-Stat3 (Tyr-705) in each group of breast cancer cells; B Immunofluorescence detects the nuclear translocation of p-Stat3 (Tyr-705) in each group of breast cancer cells treated with Quercetin; C Immunofluorescence detects the nuclear translocation of p-Stat3 (Tyr-705) in each group of breast cancer cells treated with EET, indicates P < 0.01 compared to the 0 μM group, indicates P < 0.0001; D MTS assay detects the cell viability of each group of breast cancer cells; E CCK8 assay detects the proliferation ability of each group of breast cancer cells; F Flow cytometry detects the apoptosis rate of each group of breast cancer cells, **indicates P < 0.0001 compared to the Quercetin group, ####indicates P < 0.0001 compared to the Quercetin + EET group. The data are expressed as mean ± standard deviation for quantitative data, analyzed by independent sample t-test between two groups and one-way ANOVA followed by Tukey’s post-hoc test between multiple groups. Cell experiments are repeated three times

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Molecular Medicine

ISSN: 1528-3658