Fig. 2

Overexpression of RILP inhibits proliferation, migration and invasion of osteosarcoma cells. A, B Overexpression of RILP was determined by western blot assays. GAPDH was used as a loading control. C, D The proliferation level of osteosarcoma after transfected with LV-RILP or LV-Control was evaluated by CCK-8 assay. E, F The colony formation ability of osteosarcoma cells stably overexpressing RILP or not was measured by colony formation assay. G Immunofluorescence staining assay of Ki-67 in osteosarcoma cells was performed the to measure the proliferation level, scale bar: 200 μm. H–J Representative images and quantitative analysis of osteosarcoma cell migration based on wound healing assay, scale bar: 200 μm. K, L Increased expression of RILP restrained osteosarcoma cell invasion ability based on transwell assay, scale bar: 400 μm. M–O Western blot and quantitative analyses of E-cadherin, N-cadherin and Vimentin. GAPDH was used as a loading control. *P < 0.05, **P < 0.01, ***P < 0.001