Fig. 3

Silencing of RILP promotes proliferation, migration and invasion of osteosarcoma cells. A, B The protein expression level of RILP after transfected with shRILP or not were detected by western blot assays. GAPDH was used as a loading control. C, D CCK-8 assay was applied to measure the proliferation level of osteosarcoma cells after transfected with shRILP or not. E, F Colony formation assay was performed to evaluate the colony formation ability of osteosarcoma cells after transfected with shRILP or not. G Immunofluorescence staining assay of Ki-67 in osteosarcoma cells was performed the to measure the proliferation level, scale bar: 200 μm. H–J The migration ability of osteosarcoma cells stably knocking down of RILP or not was detected by wound healing assay, scale bar: 200 μm. K, L The invasion ability of osteosarcoma cells stably knocking down of RILP or not was detected by transwell assay, scale bar: 400 μm. M–O Western blot and quantitative analyses of EMT-related protein including N-cadherin, E-cadherin and Vimentin. GAPDH was used as a loading control. *P < 0.05, **P < 0.01, ***P < 0.001