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Fig. 4 | Molecular Medicine

Fig. 4

From: RILP inhibits tumor progression in osteosarcoma via Grb10-mediated inhibition of the PI3K/AKT/mTOR pathway

Fig. 4

RILP negatively regulates PI3K/AKT/mTOR pathway to restrain the proliferation, migration and invasion of osteosarcoma cells. A Heatmap illustrating RILP-related genes based on difference analysis. B KEGG enrichment analysis revealed that RILP-associated genes are mainly enriched in PI3K-AKT signaling pathway. C–E Western blot assay was performed to detect the protein expression level of PI3K, p-PI3K, AKT, p-AKT, mTOR and p-m-TOR in osteosarcoma cells stably overexpressing RILP. GAPDH was used as a loading control. F–H Western blot assay was performed to detect the protein expression level of PI3K, p-PI3K, AKT, p-AKT, mTOR and p-m-TOR in osteosarcoma cells stably knocking down RILP. GAPDH was used as a loading control. *P < 0.05, **P < 0.01

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Molecular Medicine

ISSN: 1528-3658