Skip to main content
Fig. 7 | Molecular Medicine

Fig. 7

From: RILP inhibits tumor progression in osteosarcoma via Grb10-mediated inhibition of the PI3K/AKT/mTOR pathway

Fig. 7

RILP restrains osteosarcoma progression via Grb10-mediated inhibition of PI3K/AKT/mTOR pathway. A Molecular docking techniques show that RILP can interact with Grb10. B RILP and Grb10 bind endogenously in 143B cells. C RILP and Grb10 bind exogenously in 293T cells transfected with flag-RILP and HA-Grb10 plasmid. D, E Western blot and quantitative analyses of Grb10 and p-Grb10 in RILP-upregulated cells. F, G Western blot and quantitative analyses of Grb10 and p-Grb10 in RILP-silenced cells. H–J Evaluation of p-PI3K and p-AKT in RILP-overexpressing cells after transfection of siGrb10 or siNeg. K–M Evaluation of p-PI3K and p-AKT in RILP-silenced after transfected with Grb10 overexpression plasmid or empty vector. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Molecular Medicine

ISSN: 1528-3658