Fig. 8

Increased expression of RILP inhibits tumor growth in vivo. A Tumor volume was monitored every 7 days to measure the tumor growth in vivo. B Comparison of xenograft tumor size in nude mice after injecting 143B osteosarcoma cells stably transfected with LV-Control/LV-RILP. C Tumor weight was compared between two groups. D, E Western blot and quantitative analyses of RILP, Grb10, p-Grb10, PI3K, p-PI3K, AKT, p-AKT, mTOR and p-mTOR in xenograft tumor. F IHC analysis of RILP, p-Grb10, Ki-67, p-AKT, p-mTOR for tissues of xenograft tumors. Scale bar: 200 μm. G, H Representative H&E staining images of lung tissue in two groups, the red arrows represent lung metastasis. *P < 0.05, **P < 0.01, ***P < 0.001