Fig. 1

Ferroptosis was induced by HG in hFob1.19 cells. Cells were treated with NG (17.5 mM) and HG (26.25 mM, 35 mM, 43.75 mM, respectively) for 48 h. Following the treatments, A iron ions level was measured using the FeRhonox-1 fluorescent probe. Scale bar = 20 μm. B Intracellular iron levels were assessed using a commercial assay. C MDA assay was performed. D Lipid peroxidation was detected using the 4-HNE assay kits. E C11 BODIPY 581/591 fluorescent probe was used to detect LPO. Scale bar = 20 μm. F ROS generation was determined by DHE assay kits. Scale bar = 20 μm. G Western blot analysis was carried out to assay GPX4 expression. All data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001