Fig. 2

hFob1.19 cells viability and osteogenic function were detected. Cells were treated with NG (17.5 mM) and HG (26.25 mM, 35 mM, 43.75 mM, respectively) for 48 h. Following the treatment, A cell viability by CCK-8 was assessed and the data were expressed as values relative to the NG group. B The LDH enzyme activity was quantified using LDH content kit. C The proliferation of hFob1.19 cells detected by EdU assay. Scale bar = 200 μm. D ALP activity was determined after indicated treatments and the data were expressed as values relative to the NG group. E After osteogenic differentiation for 2 weeks, mineralized extracellular matrix in hFob1.19 cells was shown by alizarin red S staining, and the data were expressed as values relative to the NG group. Scale bar = 200 μm. All data are presented as the mean ± SD of three independent experiments. ***P < 0.001, ****P < 0.0001